DYNAMICS OF MICROMORPHOMETRY OF THE WHITE PULP OF THE SPLEEN OF SEXUALLY MATURE RATS WITH EXCESSIVE CAFFEINE CONSUMPTION AND CORRECTION WITH MEXIDOL
Abstract
The aim of the study was to identify changes in the micromorphometric parameters of the white pulp of the spleen of juvenile rats with excessive caffeine intake and the use of mexidol as a possible corrector. Materials and methods: the experiment was conducted on 72 mature white male rats with an initial body weight of 130-140 g, divided into 3 groups: group 1 – control, group 2 – rats, who consumed caffeine at a dose of 120 mg/kg/day without correction, group 3 – caffeine was consumed against the background of correction with mexidol at a dose of 50 mg / kg / day. The terms of the experiment were 7, 14, 30 and 90 days. The evaluation of micromorphometric parameters was carried out on histological sections (cross sections at the level of the spleen gate). Results and discussion: excessive caffeine consumption is accompanied by an increase in the area of white pulp by 4.71% and 4.81% on the 30th and 90th days. The maximum deviations of the studied parameters of the white pulp of the spleen were recorded mainly by 30 and 90 days. At the same time, it was found that caffeine has the greatest effect on the periarterial zone of the spleen, its periarterial lymphatic vaginas and the marginal zone. Mexidol showed the maximum effect on day 90. Conclusion. The use of caffeine in an excessive dose is accompanied by the phenomenon of an increase in the area of the white pulp of the spleen and its morphological components in juvenile rats. Mexidol is able to reduce the effects of caffeine due to its antioxidant effect and the ability to influence the vascular component of the spleen with maximum effectiveness on day 90 in mature rats.
References
2. Опасные стимуляторы организма – энергетики. URL: https://04.rospotrebnadzor.ru/index.php/consumer-information/faq/15953-14012022.html
3. Reef Al.T., Ghanem E. Caffeine: Well-known as psychotropic substance, but little as immunomodulatory.Immunobiology. 2018; 12: 818-825.
4. Horrigan L.A., Kelly J.P., Connor T.J. Immunomodulatory effects of caffeine: friend or foe? Pharmacology & Therapeutics. 2006; 111 (3): 877-892.
5. Steiniger B.S. Human spleen microanatomy: why mice do not suffice. Immunology. 2015; 145 (3): 334-346.
6. Грищенко А.А. Некоторые показатели органометрии селезенки ювенильных крыс на фоне избыточного потребления кофеина при возможной коррекции мексидолом. Морфологический альманах имени В.Г. Ковешникова. 2022; 3: 30-33.
7. Беляков В.И., Инюшкина Е.М., Громова Д.С. Лабораторные крысы: содержание, разведение и биоэтические аспекты использования в экспериментах по физиологии поведения: учебное пособие. Самара: Издательство Самарского университета. 2021. 96.
8. Толстенко А.А. Макро-микроморфометрическое исследование почек крыс различного возраста с избыточным потреблением кофеина при возможной коррекции мексидолом. Морфологический альманах имени В.Г. Ковешникова. 2023; 1: 111-115.
9. Шеламова М.А., Инсарова Н.И., Лещенко В.Г. Статистический анализ медико-биологических данных с использованием программы EXCEL: учеб. – метод. пособие. Минск: БГМУ. 2010. 96.
10. Lewis S.M., Williams A., Eisenbarth S.C. Structure and function of the immune system in the spleen. Science Immunology. 2014; 33: 6085.
11. Воронина Т.А. Мексидол: спектр фармакологических эффектов. Журнал неврологии и психиатрии. 2012; 12 (2): 86-90.
12. Грищенко А.А. Показатели макро-микроморфометрии селезенки крыс различного возраста на фоне избыточного потребления кофеина при возможной коррекции мексидолом. Проблемы экологической и медицинской генетики и клинической иммунологии. 2022; 5 (173): 31-39.
13. Leonard T.K., Watson R.R., Mohs M.E. The effects of caffeine on various body systems: a review. Journal of the American Dietetic Association.1987; 87 (8): 1048-1053.
14. Машковский М. Д. Лекарственные средства. 15-е изд. М.: Новая Волна. 2005. 1200.
