STUDY OF ANTI-INFLAMMATORY CYTOKINES – INTERLEUKIN-4 AND INTERLEUKIN-10 IN WOMEN WITH STRESS URINARY INCONTINENCE IN POSTMENOPAUSE
Abstract
The aim of the work was to assess the serum levels of anti-inflammatory cytokines – interleukin-4 and interleukin-10, in postmenopausal women with stress urinary incontinence. The study group included 150 postmenopausal women suffering from stress urinary incontinence. The control group consisted of 57 relatively healthy women of the same age. In all patients, the concentrations of interleukin-4 and interleukin-10 in blood serum were determined using the enzyme immunoassay method. The median, 25% and 75% quartiles [Q1; Q3] were calculated. The Wilcoxon W-test was used to compare the central tendencies of two independent samples. The level of statistical significance was taken as p<0.05. A comparative analysis of the central trends in the levels of the studied cytokines in the blood serum of the examined women showed that patients with stress urinary incontinence significantly differed from the control group in reduced values of interleukin 10 (p=0,023). Along with this, there were no statistically significant differences between the two groups of women in serum levels of interleukin-4. The results obtained reflect important pathogenetic aspects of the development of stress urinary incontinence in postmenopausal women and indicate the possibility of using the serum level of interleukin-10 as a potential biomarker for the diagnosis and therapeutic monitoring of stress urinary incontinence in postmenopausal women.
References
2. Касян Г.Р., Гвоздев М.Ю., Коноплянников А.Г., Пушкарь Д.Ю. Недержание мочи у женщин: методические рекомендации № 4. Москва: АБВ-пресс; 2017. 45.
3. Игнатенко Г.А., Майлян Э.А., Немсадзе И.Г., Румянцева З.С., Чурилов А.В., Глазков И.С., Мирович Е.Д. Роль цитокинов в ремоделировании костной ткани в норме и патологии. Таврический медико-биологический вестник. 2020; 23 (1): 133-139.
4. Игнатенко Г.А., Немсадзе И.Г., Мирович Е.Д., Чурилов А.В., Майлян Э.А., Глазков И.С., Румянцева З.С. Роль цитокинов в ремоделировании костной ткани и патогенезе постменопаузального остеопороза. Медицинский вестник Юга России. 2020; 11 (2): 6-18.
5. Майлян Э.А., Чурилов А.В., Немсадзе И.Г., Румянцева З.С., Глазков И.С. Цитокиновый статус женщин с постменопаузальным остеопорозом в динамике лечения алендронатом. Крымский журнал экспериментальной и клинической медицины. 2021; 11 (1): 5-11.
6. Майлян Э.А., Резниченко Н.А., Игнатенко Г.А. Сывороточные уровни цитокинов при постменопаузальном остеопорозе. Крымский журнал экспериментальной и клинической медицины. 2018; 8 (1): 36-42.
7. Поворознюк В.В., Резниченко Н.А., Майлян Э.А. Роль иммунных факторов в патогенезе постменопаузального остеопороза. Проблемы остеологии. 2013; (16) 3: 3-7.
8. Huang W.Y., Hsin I.L., Chen D.R. Circulating interleukin-8 and tumor necrosis factor-α are associated with hot flashes in healthy postmenopausal women. PLoS One. 2017; 12 (8): e0184011.
9. Ginaldi L., De Martinis M. Osteoimmunology and Beyond. Curr Med Chem. 2016; 23 (33): 3754-3774.
10. Симрок В.В., Мирович Е.Д., Золотухин С.Е., Ткаченко К.Е., Костецкая Н.И., Майлян Д.Э. Цитокиновый профиль у женщин с климактерическим синдромом и остеопорозом. Проблемы экологической и медицинской генетики и клинической иммунологии. 2022; 2: 96-103.
11. Lorenz TK, Heiman JR, Demas GE. Sexual activity modulates shifts in TH1/TH2 cytokine profile across the menstrual cycle: an observational study. Fertil Steril. 2015; 104 (6): 1513-21.e214. doi: 10.1016/j.fertnstert.2015.09.001
12. Lorenz T.K., Demas G.E., Heiman J.R. Partnered sexual activity moderates menstrual cycle-related changes in inflammation markers in healthy women: an exploratory observational study. Fertil Steril. 2017; 107 (3): 763-773.
13. Truzzi J.C., Gomes C.M., Bezerra C.A. Overactive bladder – 18 years – Part I. Int Braz J Urol. 2016; 42 (2): 188-198.
14. Pillalamarri N., Shalom D.F., Pilkinton M.L., Winkler H.A., Chatterjee P.K., Solanki M., Metz C.N. Inflammatory Urinary Cytokine Expression and Quality of Life in Patients With Overactive Bladder. Female Pelvic Med Reconstr Surg. 2018; 24 (6): 449-453.
15. Tyagi P., Barclay D., Zamora R., Yoshimura N., Peters K., Vodovotz Y., Chancellor M. Urine cytokines suggest an inflammatory response in the overactive bladder: a pilot study. Int Urol Nephrol. 2010; 42 (3): 629-635.
16. Jiang Y.H, Jhang J.F., Hsu Y.H., Ho H.C., Wu Y.H., Kuo H.C. Urine biomarkers in ESSIC type 2 interstitial cystitis/bladder pain syndrome and overactive bladder with developing a novel diagnostic algorithm. Sci Rep. 2021; 11 (1): 914.
17. Saraiva M., O’Garra A. The regulation of IL-10 production by immune cells. Nat. Rev. Immunol. 2010; 10: 170-181.
18. Rossato M., Cencig S., Gasperini S., Cassatella M.A., Bazzoni F. IL-10 modulates cytokine gene transcription by protein synthesis-independent and dependent mechanisms in lipopolysaccharide-treated neutrophils. Eur. J. Immunol. 2007; 37: 3176-3189.
19. Oguchi T., Funahashi Y., Yokoyama H., Nishizawa O., Goins W.F., Goss J.R. et al. Effect of herpes simplex virus vector-mediated interleukin-4 gene therapy on bladder overactivity and nociception. Gene Ther. 2013; 20 (2): 194-200.
20. Ma E., Vetter J., Bliss L., Lai H.H., Mysorekar I.U., Jain S. A multiplexed analysis approach identifies new association of inflammatory proteins in patients with overactive bladder. Am J Physiol Renal Physiol. 2016; 311 (1): 28-34.
